Date
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Laboratory
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3.10
2023
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GI tract
Gastric Juice - composition, production of HCl, clinical relevance (hypersecretion, achlorhydria), H.pylori infection
Practical part:
H.pylori test
Uffelman test
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10.10
2023
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Proteins
Serum proteins. Hypo-, hyper-, dysproteinemia - definitions, causes.
The functions of serum proteins. The complement system.
Clotting cascade proteins.
Intrinsic and extrinsic pathway of blood coagulation.
Practical part:
Determination of total protein concentration - standard curve.
Determination of protein concentration by Exton Method.
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17.10
2023
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Alcohol and narcotic substances
Alcohol and drugs metabolism in the body. The effects of abuse.
The practical part:
Drug test, alcohol concentration in blood.
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24.10
2023
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Enzymes and coenzymes
The kinetics of the enzymatic reaction.
Factors affecting the rate of the enzymatic reaction.
Methods of determination enzyme activity.
Enzyme inhibition.
Units of enzyme activity.
The practical part:
The kinetics of the enzymatic reaction.
Enzymes quality reactions.
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7.11
2023
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Biochemical markers in various disease states
Isolation and purification of the enzyme from the biological material.
Characteristics of selected enzymes clinically important. Characteristics of selected non-enzymatic markers.
Practical part:
Determination of the activity of selected clinically important enzymes.
Quantitative methods for determination of selected non-enzymatic markers.
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14.11.
2023
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Vitamins
The chemical structure, the source for the body, role in the metabolism, the daily demand, causes and symptoms of hypovitaminosis B1, B2, B6
and C.
Methods of determination and detection, and dynamic load tests. Methods of laboratory diagnostics in the assessment of vitamin: FIGLU test, Xanthurenic acid, Schilling test.
Practical part:
Detection and measurement of the concentration of selected vitamins.
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21.11
2023
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Calcium, phosphorus, magnesium and vitamin D
The role of calcium, phosphorus and magnesium in the body.
The presence of calcium and phosphorus in dietary products.
The daily demands.
The causes of hyper- and hypocalcemia.
The causes of hyper- and hypophosphatemia.
Calcium and phosphorus in the blood serum - fractions.
Laboratory diagnosis of calcium-phosphate homeostatis. Vitamin D in homeostasis of calcium and phosphate.
Practical part:
Determination of the concentration of calcium and inorganic phosphate.
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28.11.
2023
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Iron and trace elements
Biochemical function, sources, functions in metabolism, causes and symptoms of deficiency: selenium, iodine,copper, zinc, manganese.
Laboratory methods used in detection of trace elements.
Practical part:
Determination of the iron concentration.
Determination of the selenium concentration.
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5.12
2023
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Immunological methods of protein determination
Methods of protein separation.
Electrophoresis - the principle of the method, media variations, applications.
Immunological techniques for determination of protein concentration.
ELISA - principle of the method, applications.
Practical part:
Determination of VCAM-1 concentration by ELISA
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12.12
2023
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Hormones and tumor markers
Endocrinology tests, free and total fractions of hormones, metabolites of hormones, specialized tests: urinary excretion of 5-HIAA, the 24-hour urine collection for catecholamines and hormone metabolites.
Practical part:
Pregnancy test.
Determination of 5-HIAA.
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09.01
2024
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Reactive Oxygen Species
Reactive oxygen species – characteristics. ROS in the regulation of physiological processes.
The practical part:
MDA test in human plasma
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16.01
2024
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Rekakes
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23.01
2024
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Retakes - Comprehensive
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