4.03.2024

1. Introduction to laboratory techniques

1. 1. Use of pipette
   2. Spectroscopy/photometry
   3. Dilutions

11.03.2024 

2. Nucleic acids and molecular biology techniques

1. 1. Isolation of DNA from blood sample
   2. PCR, PCR-RFLP, PCR-STR, RT-PCR, real-time PCR
   3. Agarose and polyacrylamide gels
   4. Electrophoresis of nucleic acids

18.03.2024  

3. Serum proteins

1. Serum protein electrophoresis.
2. Hyperproteinemia and hypoproteinemia,
3. Monoclonal and polyclonal gammopathy,
4. Acute phase proteins.

8.04.2024   

4. Clinically important enzymes

1. 1. Lactate dehydrogenase (LDH), Alkaline phosphatase (AP), Acid phosphatase (ACP), Prostatic acid phosphatase (ACPP, ACP3), Tartrate-resistant acid phosphatase (ACP5), glutamic-pyruvic transaminase (ALT, SGPT), serum glutamic-oxaloacetic transaminase (AST, SGOT), Gamma-glutamyl transpeptidase (GGT), Creatine kinase (CK), amylase, lipase
   2. Non-enzymatic markers: Prostate specific antigen (PSA), Cardiac troponin I (cTnI) and T (cTnT)

15.04.2024

Colloquium I (from lectures and seminars) - details in the Lectures section (15th of April)

22.04.2024

5.Hormones: Endocrinology tests, free and total fractions of hormones, metabolites of hormones, specialized tests: urinary excretion of 5-HIAA, the 24-hour urine collection for catecholamines and hormone metabolites. Immunoenzymatic techniques, Western Blot. 

6.05.2024

6.  Estimation of fasting and postprandial blood glucose, Oral glucose tolerance test (OGTT), glycated hemoglobin. Definition and classification of diabetes mellitus, impaired glucose tolerance (IGT), impaired fasting glucose (IFG).

13.05.2024

7. Lipids: Total cholesterol, LDL, HDL, triglycerides.

1. Laboratory criteria of dyslipidemia, Friedewald formula. Primary and secondary lipid disorders.
   
2. Lipids: electrophoresis, specialized tests: ApoB100, ApoA1, electrophoresis.

20.05.2024

8. Markers of nitrogen metabolism and kidney function: creatinine, uric acid, urea, rest nitrogen, amino acids. 

27.05.2024

Colloquium II  (from lectures and seminars) 27th of May, during lab hours

3.06.2024

9. Hemoglobin and bile pigments: metabolism, excretion, the importance in clinical diagnostic of anemia and jaundice.

1. Jaundice: hemolytic, cholestatic and hepatic.
2. Iron metabolism markers (transferrin, ferritin, total iron binding capacity [TIBC], transferrin saturation, transferrin receptors), ceruloplasmin.

 10.06.2024

10. Lab retakes.