Laboratory and Seminar

8.03.2024

BASIC PROBLEMS OF CHEMICAL ANALYSIS

Safety regulations in the biochemistry lab.
Introduction to the laboratory techniques.
Units conversion, calculations, and solving problems.

22.03.2024 

INSTRUMENTAL ANALYSIS, CHEMICAL CALCULATIONS I

Introduction to UV-VIS spectrophotometry. Lambert-Beer law. Chromatography. Mass spectrometry. Fundamentals of instrumental analysis.

Practical part:
Spectrophotometry. Preparation of the standard curve.

12.04.2024

BUFFERS, CHEMICAL CALCULATIONS II - pH-METRY

The acid-base balance of the human organism. Calculation of the pH of solutions. Buffer solutions. Henderson-Hasselbalch equation.
 
Practical part:
Preparation of buffers and measurement of their pH. Determining the buffer capacity.

26.04.2024

WATER- ELECTROLYTES BALANCE OF THE HUMAN ORGANISM

Water balance and the body's water spaces. The electrolyte composition of water compartments. Regulation of the composition and volume of the body fluids. Water and electrolyte disorders. Homeostasis of water-electrolytes in the body.

Practical part:
Determination of chloride concentration in serum, urine and cerebrospinal fluid.

17.05.2024

 ANALYSIS OF NUCLEIC ACIDS - MOLECULAR BIOLOGY TECHNIQUES

Structure of nucleic acids and their properties.
Isolation of nucleic acids from biological material.
Chemical analysis of nucleic acid preparations.
PCR - the principle of the method, application; real-time PCR
Restriction endonucleases - properties, nomenclature, application.
Electrophoresis of nucleic acids
DNA sequencing.
Genome research methods.

Practical part:
Isolating DNA from a blood sample

7.06.2024

CHARACTERISTIC REACTIONS OF ORGANIC COMPOUNDS

Chemical properties of organic compounds. Characteristic reactions of functional groups.

Practical part:
Characteristic reactions of selected functional groups and organic compounds.

14.06.2024