Laboratory

24.02

2025

CARBOHYDRATES

Laboratory diagnostics of carbohydrate metabolism - static and dynamic tests, their indications, interpretation of results.

The practical part:
Determination of glucose concentration in the blood serum by an enzymatic method.
Oral glucose tolerance test.

03.03

2025

METABOLISM OF MUSCLE DURING THE REST AND EXERCISE

Glicolysis and fatty acid metabolism in muscle. Fuel utilization in muscles at rest and during exercise.

The practical part:

Creatine kinase. Lactic acid.

10.03

2025

LIPIDS

Indications, contraindications and patient preparation to diagnostics of the plasma lipid. Calculation of LDL cholesterol with the Friedewald formula. Interpretation of the results of the laboratory test plasma lipid system. Laboratory diagnostics of dyslipoprotenemias. Electrophoresis of plasma lipoproteins.

The practical part:
Determination of total cholesterol and triglycerides in the serum by enzymatic method.
Cold flotation test.
Electrophoresis of plasma lipoproteins.

17.03.

2025

HEMOGLOBIN

Structure of hemoglobin.
Biosynthesis of hemoglobin.
Derivatives of hemoglobin.
Hemoglobinopathies.

The practical part:
Determination of hemoglobin by Drabkin method
Determination of hemoglobin by Wolff method.
Spectrometric analysis of the hemoglobin derivatives.

24.03

 2025

BILE PIGMENTS; PORPHYRINS

Bilirubin - chemical structure, biosynthesis, metabolism, excretion.
Heme biosynthesis.
Congenital metabolic blocks in the biosynthesis of heme.

 The practical part:
Determination of bilirubin in the blood.
Determination of the concentration of delta-aminolevulinic acid in the blood.

31.03

2025

UREA, REST NITROGEN, URIC ACID, CREATININE

Rest nitrogen- classification, diagnostic utility, diagnostic methods.

Creatinine metabolism.

The practical part:

Creatinine clearance. Interpretation of results.

Determination of the concentration of urea and uric acid in the blood serum.

07.04

2025

ANALYSIS OF URINE part I

Physical and chemical properties of urine.

The practical part:
Analysis of urine.  
Evaluation of physical properties of urine.
Detection of acetone.
Detection of acetoacetic acid.
Detection of urobilinogen.
Detection of bile acids.
Detection of glucose.
Determination of protein concentration.

14.04

2025

ANALYSIS OF URINE part II

Please bring 4 samples of morning urine for examination.

Physical and chemical properties of urine. Guthri test.

Microscopic examination of urine sediment. Identifying the components of organized and disorganized urine sediment.
Nephrolithiasis - examination and recognition of stones.

05.05

2025

RETAKES

12.05

2025

FINAL LABORATORY EXAM - lab and theory parts- term 1

26.05

2025