Date
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Laboratory
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5.10
2026
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BASIC PROBLEMS OF CHEMICAL ANALYSIS
Safety regulations in the biochemistry lab.
Introduction to the laboratory techniques.
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12.10
2026
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INSTRUMENTAL ANALYSIS, CHEMICAL CALCULATIONS I
Units conversion, calculations, and solving problems.
Introduction to UV-VIS spectrophotometry. Lambert-Beer law. Chromatography. Mass spectrometry. Fundamentals of instrumental analysis.
Practical part:
Spectrophotometry. Preparation of the standard curve.
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19.10
2026
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BUFFERS, CHEMICAL CALCULATIONS II - pH-METRY
The acid-base balance of the human organism. Calculation of the pH of solutions. Buffer solutions. Henderson-Hasselbalch equation.
Practical part:
Preparation of buffers and measurement of their pH. Determining the buffer capacity.
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26.10
2026
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PROTEINS
Serum proteins. Hypo-, hyper-, dysproteinemia - definitions, causes.
The functions of serum proteins. The complement system.
Clotting cascade proteins.
Intrinsic and extrinsic pathway of blood coagulation.
Practical part:
Determination of total protein concentration - standard curve.
Determination of protein concentration by Exton Method.
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02.11
2026
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HEMOGLOBIN
Structure of hemoglobin.
Biosynthesis of hemoglobin.
Derivatives of hemoglobin.
Hemoglobinopathies.
The practical part:
Determination of hemoglobin by Drabkin method
Determination of hemoglobin by Wolff method.
Spectrometric analysis of the hemoglobin derivatives.
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9.11.
2026 |
ENZYMES AND COENZYMES
The kinetics of the enzymatic reaction.
Factors affecting the rate of the enzymatic reaction.
Methods of determination enzyme activity.
Enzyme inhibition.
Units of enzyme activity.
The practical part:
The kinetics of the enzymatic reaction.
Enzymes quality reactions.
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16.11
2026
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BIOCHEMICAL MARKERS IN VARIOUS DISEASE STATES
Isolation and purification of the enzyme from the biological material.
Characteristics of selected enzymes clinically important. Characteristics of selected non-enzymatic markers.
Practical part:
Determination of the activity of selected clinically important enzymes.
Quantitative methods for determination of selected non-enzymatic markers.
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23.11
2026
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VITAMINS
The chemical structure, the source for the body, role in the metabolism, the daily demand, causes and symptoms of hypovitaminosis B1, B2, B6
and C.
Methods of determination and detection, and dynamic load tests. Methods of laboratory diagnostics in the assessment of vitamin: FIGLU test, Xanthurenic acid, Schilling test.
Practical part:
Detection and measurement of the concentration of selected vitamins.
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30.11.
2026
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CALCIUM, PHOSPHORUS, MAGNESIUM AND VITAMIN D
The role of calcium, phosphorus and magnesium in the body.
The presence of calcium and phosphorus in dietary products.
The daily demands.
The causes of hyper- and hypocalcemia.
The causes of hyper- and hypophosphatemia.
Calcium and phosphorus in the blood serum - fractions.
Laboratory diagnosis of calcium-phosphate homeostatis. Vitamin D in homeostasis of calcium and phosphate.
Practical part:
Determination of the concentration of calcium and inorganic phosphate.
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07.12
2026
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IRON AND TRACE ELEMENTS
Biochemical function, sources, functions in metabolism, causes and symptoms of deficiency: selenium, iodine,copper, zinc, manganese.
Laboratory methods used in detection of trace elements.
Practical part:
Determination of the iron concentration.
Determination of the selenium concentration.
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14.12
2026
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WATER- ELECTROLYTES BALANCE OF THE HUMAN ORGANISM
Water balance and the body's water spaces. The electrolyte composition of water compartments. Regulation of the composition and volume of the body fluids. Water and electrolyte disorders. Homeostasis of water-electrolytes in the body.
Practical part:
Determination of chloride concentration in serum, urine and cerebrospinal fluid.
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11.01
2027
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IMMUNOLOGICAL METHODS OF PROTEIN DETERMINATION
Methods of protein separation.
Electrophoresis - the principle of the method, media variations, applications.
Immunological techniques for determination of protein concentration.
ELISA - principle of the method, applications.
Practical part:
Determination of VCAM-1 concentration by ELISA
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18.01
2027
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HORMONES AND TUMOR MARKERS
Endocrinology tests, free and total fractions of hormones, metabolites of hormones, specialized tests: urinary excretion of 5-HIAA, the 24-hour urine collection for catecholamines and hormone metabolites.
Practical part:
Pregnancy test.
Determination of 5-HIAA.
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25.01
2027
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Retakes
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01.02
2027
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Retakes - Comprehensive
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