8.10.2024

1. Introduction to laboratory techniques

1. 1. Use of pipette
   2. Spectroscopy/photometry
   3. Dilutions

15.10.2024 

2. Nucleic acids and molecular biology techniques

1. 1. Isolation of DNA from blood sample
   2. PCR, PCR-RFLP, PCR-STR, RT-PCR, real-time PCR
   3. Agarose and polyacrylamide gels
   4. Electrophoresis of nucleic acids

22.10.2024  

3. Serum proteins

1. Serum protein electrophoresis.
2. Hyperproteinemia and hypoproteinemia,
3. Monoclonal and polyclonal gammopathy,
4. Acute phase proteins.

5.11.2024   

4. Clinically important enzymes

1. 1. Lactate dehydrogenase (LDH), Alkaline phosphatase (AP), Acid phosphatase (ACP), Prostatic acid phosphatase (ACPP, ACP3), Tartrate-resistant acid phosphatase (ACP5), glutamic-pyruvic transaminase (ALT, SGPT), serum glutamic-oxaloacetic transaminase (AST, SGOT), Gamma-glutamyl transpeptidase (GGT), Creatine kinase (CK), amylase, lipase
   2. Non-enzymatic markers: Prostate specific antigen (PSA), Cardiac troponin I (cTnI) and T (cTnT)

12.11.2024

5.Hormones: Endocrinology tests, free and total fractions of hormones, metabolites of hormones, specialized tests: urinary excretion of 5-HIAA, the 24-hour urine collection for catecholamines and hormone metabolites. Immunoenzymatic techniques, Western Blot.

19.11.2024

 Colloquium I (from lectures and seminars) - details in the Lectures section (19th of November)

26.11.2024

6.  Estimation of fasting and postprandial blood glucose, Oral glucose tolerance test (OGTT), glycated hemoglobin. Definition and classification of diabetes mellitus, impaired glucose tolerance (IGT), impaired fasting glucose (IFG).

3.12.2024

7. Lipids: Total cholesterol, LDL, HDL, triglycerides.

1. Laboratory criteria of dyslipidemia, Friedewald formula. Primary and secondary lipid disorders.
   
2. Lipids: electrophoresis, specialized tests: ApoB100, ApoA1, electrophoresis.

10.12.2024

8. Markers of nitrogen metabolism and kidney function: creatinine, uric acid, urea, rest nitrogen, amino acids. 

7.01.2025

9. Hemoglobin and bile pigments: metabolism, excretion, the importance in clinical diagnostic of anemia and jaundice.

1. Jaundice: hemolytic, cholestatic and hepatic.
2. Iron metabolism markers (transferrin, ferritin, total iron binding capacity [TIBC], transferrin saturation, transferrin receptors), ceruloplasmin.

 14.01.2025

Colloquium II  (from lectures and seminars) 14th of January, during lab hours

 21.01.2025