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Topic
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02.03.2026
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BASIC PROBLEMS OF CHEMICAL ANALYSIS, INSTRUMENTAL ANALYSIS
Safety regulations in the biochemistry lab.
Introduction to the laboratory techniques.
Introduction to UV-VIS spectrophotometry. Lambert-Beer law. Chromatography. Mass spectrometry. Fundamentals of instrumental analysis.
Practical part:
Spectrophotometry. Preparation of the standard curve.
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09.03.2026
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BUFFERS, CHEMICAL CALCULATIONS - pH-METRY
The acid-base balance of the human organism. Calculation of the pH of solutions. Buffer solutions. Henderson-Hasselbalch equation.
Practical part:
Preparation of buffers and measurement of their pH. Determining the buffer capacity.
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16.03.2026
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CHARACTERISTIC REACTIONS OF ORGANIC COMPOUNDS
Chemical properties of organic compounds. Characteristic reactions of functional groups.
Practical part:
Characteristic reactions of selected functional groups and organic compounds.
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23.03.2026
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ENZYMES AND COENZYMES
The kinetics of the enzymatic reaction.
Factors affecting the rate of the enzymatic reaction.
Methods of determination enzyme activity.
Enzyme inhibition.
Units of enzyme activity.
The practical part:
The kinetics of the enzymatic reaction.
Enzymes quality reactions.
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30.03.2026
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ANALYSIS OF NUCLEIC ACIDS - MOLECULAR BIOLOGY TECHNIQUES
Structure of nucleic acids and their properties.
Isolation of nucleic acids from biological material.
Chemical analysis of nucleic acid preparations.
PCR - the principle of the method, application; real-time PCR
Restriction endonucleases - properties, nomenclature, application.
Electrophoresis of nucleic acids
DNA sequencing.
Genome research methods.
Practical part:
Isolating DNA from a blood sample
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13.04.2026
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CALCIUM, PHOSPHORUS, MAGNESIUM AND ELECTROLYTES BALANCE OF THE HUMAN ORGANISM
Water balance and the body's water spaces. The electrolyte composition of water compartments. Regulation of the composition and volume of the body fluids. Water and electrolyte disorders. Homeostasis of water-electrolytes in the body.
The role of calcium, phosphorus and magnesium in the body.
The presence of calcium and phosphorus in dietary products.
The daily demands.
The causes of hyper- and hypocalcemia.
The causes of hyper- and hypophosphatemia.
Calcium and phosphorus in the blood serum - fractions.
Laboratory diagnosis of calcium-phosphate homeostatis. Vitamin D in homeostasis of calcium and phosphate.
Practical part:
Determination of chloride concentration in serum, urine and cerebrospinal fluid.
Determination of the concentration of calcium and magnesium.
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20.04.2026
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VITAMINS
The chemical structure, the source for the body, role in the metabolism, the daily demand, causes and symptoms of hypovitaminosis B1, B2, B6 and C.
Methods of determination and detection, and dynamic load tests. Methods of laboratory diagnostics in the assessment of vitamin: FIGLU test, Xanthurenic acid, Schilling test.
Practical part:
Detection and measurement of the concentration of selected vitamins.
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27.04.2026
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IMMUNOLOGICAL METHODS OF PROTEIN DETERMINATION
Methods of protein separation.
Electrophoresis - the principle of the method, media variations, applications.
Immunological techniques for determination of protein concentration.
ELISA - principle of the method, applications.
Practical part:
Determination of VCAM-1 concentration by ELISA
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04.05.2026
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RETAKES
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